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The study of their location was rst addressed using biochemical approaches Regardless of the class and type of holin, all chains feature a highly charged C-terminal tail Gene fusion experiments together with protease protection assays support the cytoplasmic location for the highly charged C-terminus (Bl si et al 1999; Park et al 2007) This location is conserved for most holins, and in only one, the T4 holin, has this segment been found at the periplasm (Tran et al 2005) It must be stressed that T4 holin is indeed an unusual member Its chain is twice as long as the conventional holins, and the theoretical analysis yields a single transmembrane segment together with a very long C-terminal tail (Tran et al 2005).

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Regarding the N-terminus, biochemical studies coupling extensive cysteine-scanning mutagenesis with chemical modi cation with thiolspeci c reagents showed that the active class I holin S 105 crosses the bilayer three times Similar experiments showed that the inhibitory action of S 107 became impaired on the adoption of the 3-TM topology, suggesting both a dual location in the N-terminus and the relation of this location with function (see below) Regarding the class II holins, topological studies have been mainly focused on S21 (Fig 63) Using similar strategies as before, the N- and C-terminal tails of S21 have been located to the cytoplasm in the short and long nascent forms (Park et al 2006) However, in this case, in the short form (S2168), the nascent topology evolves so the N-terminus translocates to the periplasms together with the extrusion of TM1 (Park et al 2006)..

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Most modern browsers have pop-up blockers that will stop the Open Browser Window action from functioning. although there is nothing you, as a page designer, can do to override the pop-up blocker, you might consider adding a note to your page indicating that pop-up windows are in use on your page. n

The first line in output 3.3 is the first element of @ a r r a y ,which is an example of assignment going from left to right. With this rule in mind, the output of the second p r i n t statement is no surprise. These assignments are done as if in parallel, so the third p r i n t statement reveals that the values previously in $ f i r s t and $second have been switched. Finally, ( $ f i r s t ) is an array, while $ f i r s t is a scalar. Consequently, the last p r i n t statement shows that the length of the array is stored in $ f i r s t , which differs from the first p r i n t statement.

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Command:MIRROR Select objects: (pick 1) Specify opposite corner: (pick 2) Select objects:~ Specify first point of mirroring plane:END of (pick 3) Specify second point of mirroring plane:END of (pick 4) Delete source objects [Yes/No]<N>:.J

(Ill-posedness due to wrong boundary conditions)

By inserting (7.109) and (7.1 10)into (7.108) and using the expression for the collision integral for the Maxwell gas found in Exercise 174, we obtain the following integral equations for A and B:

Geometric constraints apply to the geometric properties of objects. The basic principle is that the first object that you specify is the base object, and the second object that you specify moves or adjusts in accordance with the constraint. For example, if you have two lines at different angles and use the parallel constraint, the second line will rotate to become parallel with the base object. In most cases, you can specify a specific point on an object to manage the constraint. To specify a geometric constraint for one or more objects, display the Parametric tab and use the Geometric panel. Each button is an option of the GEOMCONSTRAINT command. Table 10.2 lists the available geometric parameters and their functions.

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You may notice that the repeating and editable regions tabs overlap, making it difficult to see the repeating region name. I ve found it handy to use fairly long names for the repeating region, such as datarowrepeating, and relatively short names for the editable region, such as datarow, to enable me to see portions of both tabs. also, although all template regions use the same color established in Preferences, the highlight for a repeating region is significantly lighter than that for editable regions. This color variation makes it much easier to identify the different types of regions. n

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